Identification and Evaluation of Regulatory Role of miR164b in Malaysian Rice Variety (MR303) under Drought Stress

MR303 is a newly released Malaysian rice cultivar that comes with special traits as it can be cultivated in less fertile soils. Previous studies stated miR164b key molecule responsible for regulating drought stress tolerance many varieties. Thus, this study aims to identify the presence and regulatory role of variety using both computational experimental approaches. The stem-loop structure (pre-miR164b) was identified through reverse-transcriptase PCR (RT-PCR) size ~ 100 bp. target prediction by psRNA revealed gene NAM protein NAC transcription factor. A expression RT-PCR followed Image J analysis control drought-treated plants demonstrated low observed sample, which led accumulation its target, NAM1. This provides preliminary knowledge variety.